This is an exciting opportunity to join a fast-paced, growing Focused Research Organization (FRO) startup (currently in stealth). We are seeking a highly motivated scientist to conduct NGS-based experimental research and contribute to novel assay development in a dynamic, interdisciplinary environment. The successful candidate will develop high-throughput workflows and generate datasets from adaptive immune cells, including bulk RNA-seq, single cell RNA-seq, long-read sequencing, BCR sequencing, and barcoded antigen labeling. This individual will work closely with other top-tier experimental and computational scientists to create groundbreaking new insights into adaptive immunity.
Responsibilities
- Aid in planning and executing NGS-based sequencing experiments to profile adaptive immune responses in coordination with interdisciplinary team of computational, immunology, and molecular biology scientists.
- Design and prepare DNA and RNA libraries from immune cells.
- Performing in-house QC checks and maintain high standards for sequencing quality, in line with industry best practices.
- Help establish and optimize sequencing workflow SOPs for NGS assays.
- Aid in maintaining sequencer uptime, and manage reagent inventory.
- Contribute to the development of novel NGS and multiomics assays and research, inclusive of experimental design.
- Assist in procurement and maintenance of supplies, chemicals, and biological inventories.
- Communicate study findings efficiently and present data at various internal and external meetings as projects progress.
- Demonstrate a commitment to diversity, inclusion, and cultural awareness through actions, interactions, and communications with others.
Key Qualifications
- BS, MS, or PhD in biomedical engineering, genetics, immunology or related field that relies on next-generation sequencing applications.
- High proficiency with nucleic acid preparation from eukaryotic sources, preferably adaptive immune cells.
- Experience with library design and preparation for next-generation sequencing.
- Familiarity with state-of-art methodology for bulk RNA-seq, single cell RNA-seq, and BCR profiling.
- Experience processing diverse single-cell raw data using Cell Ranger and alternative / open-source pipelines
- Comfort running multiple projects simultaneously with different priorities and timelines.
- Strong organizational, time management, and communication skills.
Advanced Qualifications
- Prior experience in adaptive immunity research. (strongly preferred)
- Familiarity with diverse next-generation sequencing technologies such as Pacific Biosciences, Oxford Nanopore, and/or Ion Torrent. (strongly preferred)
- Experience with single-cell multiomics techniques (eg, CITE-seq, LIBRA-seq), long-reads, and / or proteomic antibody sequencing (Ab-seq). (strongly preferred)
- Familiarity with diverse microfluidics, microwell, and microfluidics-free platforms.
- Experience with liquid handling automation for sequencing library preparation.
- Proficiency in custom protocol development.
- Experience with novel assay development.
- Competence in R and/or Python with familiarity with scVerse tools, scVi tools, Scanpy or Seurat, as well as antigen-specific analysis using scRepertoire, Immcantation, or related tools.
- Experience generating immunological insights from single cell sequencing data.
We Offer
- Competitive compensation $90,000 - $120,000 a year (salary based on years experience)
- Excellent medical, dental, and vision insurance
- Generous time off + paid holidays
- A supportive environment to learn and develop new skills
- An opportunity to participate in high-impact, fast-paced, cutting-edge, collaborative team science; contribute to curing disease; and work with leading experts from different fields
- Exciting location in the Soho area of Manhattan
