Scientist II, Assay Development & CRISPR Genome Editing

What if… you could join an organization that creates, resources, and builds life sciences companies that invent breakthrough technologies to transform human health and sustainability?

Flagship Pioneering's venture creation engine has conceived and created companies such as Moderna Therapeutics (NASDAQ: MRNA), Sana Biotechnology (NASDAQ: SANA), Generate Biomedicines, and Tessera Therapeutics. Since its launch in 2000, Flagship has applied its unique hypothesis-driven innovation process to originate and foster more than 100 scientific ventures, resulting in over $212 billion in aggregate value. To date, Flagship has deployed over $2.5 billion in capital toward the founding and growth of its pioneering companies alongside more than $19 billion of follow-on investments from other institutions. In 2021, Flagship Pioneering was ranked 12^th globally on Fortune’s “Change the World” list, an annual ranking of companies that have made a positive social and environmental impact through activities that are part of their core business strategies.

The Company:

Quotient Therapeutics, Inc. is a privately held, early-stage company developing a novel genomics platform and therapeutics for diseases with a large unmet need.

Position Summary:

We are seeking a highly skilled and motivated Ph.D. Scientist with expertise in assay development and CRISPR genome editing to join our innovative research team. The successful candidate will lead efforts to perturbate gene of interest in primary cells and cell lines, and establish assays for biological validation, contributing to the advancement of our drug discovery programs.

Responsibilities:

• Design and develop assays for target validation, including signaling pathways and molecules, biology readouts, and functional genomics.
• Design and execute experiments with various gene manipulation methods, including overexpression, knockdowns, knockouts and knockins.
• Design and develop CRISPR-based genome editing experiments to generate cell lines with specific mutations for target validation and drug screening applications.
• Characterize edited cell lines using molecular/cellular biology techniques, including PCR, sequencing, and gene expression, and functional analysis.
• Collaborate with cross-functional teams to support drug discovery projects, providing expertise in CRISPR technology and genetic engineering.
• Stay current on the latest advancements in assay development and gene perturbation technologies, and apply relevant knowledge to research projects
• Mentor research associates and junior scientists in experimental design and data analysis.

Qualifications:

• PhD. in Molecular Biology, Genetics, Biochemistry, or a related field with at least 1 year of industry experience in a biotech or pharmaceutical setting (level will be adjusted according to experience)
• Demonstrated expertise in CRISPR genome editing and genetic engineering, with a focus on introducing KO or KI on primary cells and cell lines
• Experience with mammalian cell culture techniques, including cell line maintenance, transfection, and selection of edited clones
• Proficiency in molecular biology techniques, such as PCR, cloning, and sequencing
• Strong analytical and problem-solving skills, with the ability to troubleshoot and optimize experimental protocols
• Excellent communication and collaboration skills, with the ability to work effectively in a team environment
• Prior experience in a pharmaceutical or biotechnology industry setting is preferred

Location: Cambridge, MA 

About Flagship

Flagship Pioneering is a bioplatform innovation company that invents and builds platform companies, each with the potential for multiple products that transform human health or sustainability. Since its launch in 2000, Flagship has originated and fostered more than 100 scientific ventures, resulting in more than $90 billion in aggregate value. Many of the companies Flagship has founded have addressed humanity’s most urgent challenges: vaccinating billions of people against COVID-19, curing intractable diseases, improving human health, preempting illness, and feeding the world by improving the resiliency and sustainability of agriculture.  Flagship has been recognized twice on FORTUNE’s “Change the World” list, an annual ranking of companies that have made a positive social and environmental impact through activities that are part of their core business strategies, and has been twice named to Fast Company’s annual list of the World’s Most Innovative Companies. Learn more about Flagship at www.flagshippioneering.com.

Flagship Pioneering and our ecosystem companies are committed to equal employment opportunity regardless of race, color, ancestry, religion, sex, national origin, sexual orientation, age, citizenship, marital status, disability, gender identity or Veteran status.

At Flagship, we recognize there is no perfect candidate. If you have some of the experience listed above but not all, please apply anyway. Experience comes in many forms, skills are transferable, and passion goes a long way. We are dedicated to building diverse and inclusive teams and look forward to learning more about your unique background.

Recruitment & Staffing Agencies: Flagship Pioneering and its affiliated Flagship Lab companies (collectively, “FSP”) do not accept unsolicited resumes from any source other than candidates. The submission of unsolicited resumes by recruitment or staffing agencies to FSP or its employees is strictly prohibited unless contacted directly by Flagship Pioneering’s internal Talent Acquisition team. Any resume submitted by an agency in the absence of a signed agreement will automatically become the property of FSP, and FSP will not owe any referral or other fees with respect thereto.